MyBioSource.com's Human anti-filaggrin antibody, AFA ELISA Kit is a Human monoclonal antibody. The Human anti-filaggrin antibody, AFA ELISA Kit was generated using AFA as the antigen. It reacts with Human. This antibody has been shown to work in applications such as: ELISA.
Description
Intended Uses: This sandwich kit is for the accurate quantitative detection of human anti-filaggrin antibody (also known as AFA) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human AFA antibody. AFA present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human AFA Antibody is added and binds to AFA in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated AFA antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human AFA. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm